Spata2^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Spata2^{tm1(KOMP)Vlcg}
• Name: spermatogenesis associated 2; targeted mutation 1, Velocigene
• MGI ID: MGI:3800001
• Synonyms: Spata2^tm1Vlcg
• Gene: Spata2 Location: Chr2:167323053-167334804 bp, - strand Genetic Position: Chr2, 87.23 cM
• Alliance: Spata2^{tm1(KOMP)Vlcg} page
• IMPC: Spata2 gene page

Mutation origin

• Mutant Cell Lines: 10289A-E1, 10289A-F6, 10289B-A12
• Germline Transmission: Earliest citation of germline transmission: J:244164
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2142 bp between positions 167308831-167310972 of Chromosome 2 (Genome Build37). This deletes the entire 2-exon coding region. Western blot experiments confirmed the lack of protein expression from this allele in mice, mouse embryonic fibroblasts (MEFs) and bone marrow-derived macrophages (BMDMs). (J:136110)

Expression

In Mice Carrying this Mutation:

13 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Spata2 Mutation: 20 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)