Efhd2^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Efhd2^{tm1(KOMP)Vlcg}
• Name: EF hand domain containing 2; targeted mutation 1, Velocigene
• MGI ID: MGI:3800038
• Synonyms: Efhd2^tm1Iev, Efhd2^tm1Vlcg
• Gene: Efhd2 Location: Chr4:141585453-141602231 bp, - strand Genetic Position: Chr4, 74.75 cM, cytoband E1
• Alliance: Efhd2^{tm1(KOMP)Vlcg} page
• IMPC: Efhd2 gene page

Mutation origin

• Mutant Cell Lines: 10332A-C6, 10332A-D1, 10332B-D10
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 15139bp between positions 141415641-141430779 of Chromosome 4 (Genome Build37). The lacZ reporter gene and neomycin resistance selection cassette replaced the coding sequence. Western blot analysis confirmed the absence of protein expression in bone marrow, spleen and thymus. (J:136110, J:223142, J:228565)

Expression

In Mice Carrying this Mutation:	50 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Efhd2 Mutation: 15 strains or lines available

Notes

Cell line 10332A-D1 was successfully used to make chimeric mice. Germline transmission was accomplished. J:228565

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 8 reference(s)