Rnf8^{Gt(RRR260)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Rnf8^{Gt(RRR260)Byg}
• Name: ring finger protein 8; gene trap RRR260, BayGenomics
• MGI ID: MGI:3800229
• Synonyms: RNF8^-
• Gene: Rnf8 Location: Chr17:29833763-29860638 bp, + strand Genetic Position: Chr17, 15.45 cM
• Alliance: Rnf8^{Gt(RRR260)Byg} page

Impaired spermatogenesis in Rnf8^{Gt(AS0574)Wtsi}/Rnf8^{Gt(AS0574)Wtsi} and Rnf8^{Gt(RRR260)Byg}/Rnf8^{Gt(RRR260)Byg} males

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: RRR260
• Germline Transmission: Earliest citation of germline transmission: J:137023
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector is inserted after exon 4, disrupting proper transcription of the gene and generating a fusion product of beta-geo and the N-terminal part of the encoded protein without its C-terminal RING domain which is critical for function. The absence of protein expression was confirmed by western blot analysis on thymocyte extracts. (J:137023, J:159114, J:161240)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR260	CG784935 ; (MGI Seq Detail)	5' RACE	Chr17:29846096-29847993 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Rnf8^{Gt(AS0574)Wtsi} and Rnf8^{Gt(RRR260)Byg} alleles

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf8 Mutation: 21 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:137023 Minter-Dykhouse K, et al., Distinct versus overlapping functions of MDC1 and 53BP1 in DNA damage response and tumorigenesis. J Cell Biol. 2008 Jun 2;181(5):727-35
• All: 16 reference(s)