Pfdn1^{Gt(GTR1.3)1Rul}
Gene trapped Allele Detail

Summary

• Symbol: Pfdn1^{Gt(GTR1.3)1Rul}
• Name: prefoldin 1; gene trap 1, H Earl Ruley
• MGI ID: MGI:3800373
• Gene: Pfdn1 Location: Chr18:36536732-36587548 bp, - strand Genetic Position: Chr18, 19.46 cM
• Alliance: Pfdn1^{Gt(GTR1.3)1Rul} page

Mutation origin

• Mutant Cell Line: b3p1a6
• Germline Transmission: Earliest citation of germline transmission: J:137415
• Parent Cell Line: AC1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGTR1.3
• Mutation details: The retrovirus gene trap vector GTR1.3 containing a puromycin gene, a lacZ gene, a Pol2 gene and a neo cassette was inserted into intron 3. The absence of protein product was confirmed by western blot analysis on embryonic fibroblast extracts. Northern blot analysis confirmed the expression of a transcript of increased size in embryonic fibroblasts. (J:137415, J:137511)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  PSTVUpb4a4	CZ169616 ; (MGI Seq Detail)	3' RACE	Chr18:36537141-36537514 bp (-)
  PSTVUpb7f2	CZ169816 ; (MGI Seq Detail)	3' RACE	unknown
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  608 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pfdn1 Mutation: 173 strains or lines available

References

• Original: J:137415 Cao S, et al., Subunit 1 of the prefoldin chaperone complex is required for lymphocyte development and function. J Immunol. 2008 Jul 1;181(1):476-84
• All: 2 reference(s)