Igh^tm1Rbr
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Rbr
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Robert Brink
• MGI ID: MGI:3800383
• Synonyms: VH-10tar
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132538
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted
• Mutations: Deletion, Insertion
• Igh^tm1Rbr involves 1 genes/genome features (Igh) View all
• Mutation details: The HyHEL10 hybridoma produces an antibody that binds hen egg lysozyme peptide (HEL) with high affinity. The rearranged variable heavy gene segment from the HyHEL10 hybridoma replaced the endogenous J gene segments of the IgH locus. A floxed neomycin cassette was placed upstream for targeting purposes. About 0.4% of B cells produce antibody that bind HEL with high affinity. (J:132538)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:132538 Phan TG, et al., B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells. J Exp Med. 2003 Apr 7;197(7):845-60
• All: 17 reference(s)