Bcl2l11^tm2Rjd
Targeted Allele Detail

Summary

• Symbol: Bcl2l11^tm2Rjd
• Name: BCL2 like 11; targeted mutation 2, Roger J Davis
• MGI ID: MGI:3800457
• Synonyms: Bim^3SA
• Gene: Bcl2l11 Location: Chr2:127967958-128004467 bp, + strand Genetic Position: Chr2, 62.23 cM, cytoband F3-G1
• Alliance: Bcl2l11^tm2Rjd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137061
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A floxed neo cassette was inserted within intron 4, and specific mutations in exon 3 were introduced by homologous recombination. Mutations resuted in three sites of MAPK phosphorylation (Ser-55, Ser-65, and Ser-73) in exon 3 to be substituted with alanines (S55A, S65A, S73A). The neo cassette was then excised with cre recombinase leaving a single loxP site in intron 4. Expression of normal amounts of mutant proteins was confirmed by immunoblot. (J:137061)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bcl2l11 Mutation: 36 strains or lines available

References

• Original: J:137061 Hubner A, et al., Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell. 2008 May 23;30(4):415-25
• All: 3 reference(s)