Bcl2l11^tm4Rjd
Targeted Allele Detail

Summary

• Symbol: Bcl2l11^tm4Rjd
• Name: BCL2 like 11; targeted mutation 4, Roger J Davis
• MGI ID: MGI:3800472
• Synonyms: Bim^deltaEL, BimL-only
• Gene: Bcl2l11 Location: Chr2:127967958-128004467 bp, + strand Genetic Position: Chr2, 62.23 cM, cytoband F3-G1
• Alliance: Bcl2l11^tm4Rjd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137061
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted within intron 4, and genomic sequence with deleted alternatively spliced exon 3 was introduced by homologous recombination. Replaced mutation caused the entire exon 3, necessary for BimEL isoform transcription, to be deleted. The neo cassette was then excised with cre recombinase, leaving a single loxP site in intron 4. By immunoblot analyses, no BimEL proteins were expressed; however, increased amounts of BimL proteins was expressed. This is BimEL isoform specific knock-out allele. (J:137061)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bcl2l11 Mutation: 36 strains or lines available

References

• Original: J:137061 Hubner A, et al., Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell. 2008 May 23;30(4):415-25
• All: 3 reference(s)