Chd2^{Gt(RRBO46)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Chd2^{Gt(RRBO46)Byg}
• Name: chromodomain helicase DNA binding protein 2; gene trap RRB046, BayGenomics
• MGI ID: MGI:3800602
• Synonyms: Chd2^m, Chd2^mut
• Gene: Chd2 Location: Chr7:73076400-73191494 bp, - strand Genetic Position: Chr7, 41.85 cM
• Alliance: Chd2^{Gt(RRBO46)Byg} page

Mutation origin

• Mutant Cell Line: RRB046
• Germline Transmission: Earliest citation of germline transmission: J:146743
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector containing a lacZ and neo cassette inserted into intron 27. RT-PCR confirmed the lack of trancripts containing exons 26 through 29, and western blot analysis confirmed the expression of the beta-galactosidase fusion protein. (J:146743)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRB046	CC183269 ; (MGI Seq Detail)	5' RACE	Chr7:73113385-73121618 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  167 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	20 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chd2 Mutation: 190 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:146743 Marfella CG, et al., Mutation of the SNF2 family member Chd2 affects mouse development and survival. J Cell Physiol. 2006 Oct;209(1):162-71
• All: 5 reference(s)