Drosha^tm1Litt
Targeted Allele Detail

Summary

• Symbol: Drosha^tm1Litt
• Name: drosha, ribonuclease type III; targeted mutation 1, Dan R Littman
• MGI ID: MGI:3801076
• Synonyms: Drosha^F, Drosha^flEx9, Drosha^lox
• Gene: Drosha Location: Chr15:12824901-12935377 bp, + strand Genetic Position: Chr15, 5.99 cM
• Alliance: Drosha^tm1Litt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138683
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was used to introduce a loxP-flanked neomycin resistance cassette to intron 8, and an additional loxP site to intron 9 of the gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The neomycin cassette was excised with a C57BL/6 background EIIa-Cre, leaving loxP sites on either side of exon 9. (J:138683)

Expression

In Mice Carrying this Mutation:	5 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Drosha Mutation: 96 strains or lines available

References

• Original: J:138683 Chong MM, et al., The RNAseIII enzyme Drosha is critical in T cells for preventing lethal inflammatory disease. J Exp Med. 2008 Sep 1;205(9):2005-17
• All: 25 reference(s)