Gt(ROSA)26Sor^tm1Joe
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Joe
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Jonathan A Epstein
• MGI ID: MGI:3801312
• Synonyms: GFP-NLS-lacZ, GNZ, ROSA26-GNZ, ROSA-nGFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1Joe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135149
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed with a loxP-flanked STOP cassette (PGK-Neo-polyA followed by transcriptional stop sequence) just upstream of a green fluorescent protein/beta-galactosidase fusion protein sequence with SV40 nuclear localization signal (GFP-NLS-lacZ or GNZ). This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation. (J:135149)

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:135149 Stoller JZ, et al., Cre reporter mouse expressing a nuclear localized fusion of GFP and beta-galactosidase reveals new derivatives of Pax3-expressing precursors. Genesis. 2008 Apr;46(4):200-4
• All: 23 reference(s)