Wt1^{tm2(cre/ERT2)Wtp}
Targeted Allele Detail

Summary

• Symbol: Wt1^{tm2(cre/ERT2)Wtp}
• Name: WT1 transcription factor; targeted mutation 2, William T Pu
• MGI ID: MGI:3801682
• Synonyms: Wt1^CreERT2, Wt1^iCre, Wt1^{tm2(cre/ESR1)Wtp}
• Gene: Wt1 Location: Chr2:104956874-105003959 bp, + strand Genetic Position: Chr2, 55.06 cM, cytoband E
• Alliance: Wt1^{tm2(cre/ERT2)Wtp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137426
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Wt1^{tm2(cre/ERT2)Wtp} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Wt1 (MGI:98968)

• Mutation details: Exon 1 was replaced with an inducible cre ESR1 (ERT2) fusion followed by an FRT-flanked neo cassette. Testing in 2023 by the investigator's laboratory, indicates that the FRT-flanked neo has not been removed as reported. (J:137426)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Wt1 (mouse) Summary of all recombinase alleles driven by Wt1. Comparative matrix view of recombinase activities Alliance mouse Wt1 gene page

Disease models

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wt1 Mutation: 34 strains or lines available

References

• Original: J:137426 Zhou B, et al., Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature. 2008 Jul 3;454(7200):109-13
• All: 89 reference(s)