Tg(IGH@yH1)2BJkbv
Transgene Detail

Summary

• Symbol: Tg(IGH@yH1)2BJkbv
• Name: transgene insertion 2B, Aya Jakobovits
• MGI ID: MGI:3802657
• Synonyms: HuAb-mu, Tg(IGHyH1)2BJkbv, yH1
• Transgene: Tg(IGH@yH1)2BJkbv Location: unknown
• Alliance: Tg(IGH@yH1)2BJkbv page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:23827
• Parent Cell Line: E14.1TG3B1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IGH@yH1)2BJkbv expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  human	IGH (3492)

• Mutation details: A yeast artificial chromosome was constructed with a 220-kb insert of the human IgH locus. This insert contained in germline configuration the mu and delta constant regions, the intronic enhancer, all six functional J-h regions, the entire D complex, and the five most proximal variable gene segments. An HPRT mini-gene was included downstream of the constant regions to aid targeting. Line 2B had one copy of the YAC intergrate into the mouse genome and was one of 7 lines generated. Southern blotting confirmed that the YAC integrated without any recombination events occurring. (J:23827)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:23827 Green LL, et al., Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs. Nat Genet. 1994 May;7(1):13-21
• All: 3 reference(s)