Tg(IGK@yK1)9.2CJkbv
Transgene Detail
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Symbol: |
Tg(IGK@yK1)9.2CJkbv |
Name: |
transgene insertion 9.2C, Aya Jakobovits |
MGI ID: |
MGI:3802658 |
Synonyms: |
HuAb-kappa, Tg(IGKyK1)9.2CJkbv, yK1 |
Transgene: |
Tg(IGK@yK1)9.2CJkbv Location: unknown
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Alliance: |
Tg(IGK@yK1)9.2CJkbv page
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Germline Transmission: |
Earliest citation of germline transmission:
J:23827
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Parent Cell Line: |
E14.1TG3B1 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(IGK@yK1)9.2CJkbv expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Note |
human |
IGK (50802) |
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Mutation details: A yeast artificial chromosome was constructed with a 170-kb insert of the human IgK locus. This insert contained in germline configuration the kappa deleting element, the intronic and downstream enhancers, all five functional J-k regions, and the three most proximal variable gene segments. An HPRT mini-gene was included downstream of the constant regions to aid targeting. Line 9.2C had one copy of the YAC intergrate into the mouse genome and was one of at least 8 lines generated. Southern blotting confirmed that the YAC integrated without any recombination events occurring.
(J:23827)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Original: |
J:23827 Green LL, et al., Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs. Nat Genet. 1994 May;7(1):13-21 |
All: |
3 reference(s) |
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