Tg(IGH@yH2)J9.2Jkbv
Transgene Detail

Summary

• Symbol: Tg(IGH@yH2)J9.2Jkbv
• Name: transgene insertion J9.2, Aya Jakobovits
• MGI ID: MGI:3802713
• Synonyms: Tg(IGHyH2)J9.2Jkbv, yH2
• Transgene: Tg(IGH@yH2)J9.2Jkbv Location: unknown
• Alliance: Tg(IGH@yH2)J9.2Jkbv page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:70620
• Parent Cell Line: E14.1TG3B1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IGH@yH2)J9.2Jkbv expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  human	IGH (3492)

• Mutation details: A 970-kb yeast artificial chromosome (YAC) was constructed from human germline sequence of the immunoglobulin heavy chain gene cluster. This YAC consisted of 66 V-heavy gene segments, all 6 J segments, all 30 D gene segments, the C-mu, the C-delta and the C-gamma2 constant regions, a downstream murine enhancer element and a neomycin resistance cassette. Seven transgenic lines were created with two, L10 and J9.2, being propagated for further analysis. Southern blot analysis confirmed that the YAC inserted without any recombination events. Transgenic mice were found to express human heavy chain immunoglobulin. (J:70620, J:139719)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:70620 Mendez MJ, et al., Functional transplant of megabase human immunoglobulin loci recapitulates human antibody response in mice. Nat Genet. 1997 Feb;15(2):146-56
• All: 6 reference(s)