Tg(IGK@yK2)J23.1Jkbv
Transgene Detail

Summary

• Symbol: Tg(IGK@yK2)J23.1Jkbv
• Name: transgene insertion J23.1, Aya Jakobovits
• MGI ID: MGI:3802714
• Synonyms: Tg(IGKyK2)J23.1Jkbv, yK2
• Transgene: Tg(IGK@yK2)J23.1Jkbv Location: unknown
• Alliance: Tg(IGK@yK2)J23.1Jkbv page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:70620
• Parent Cell Line: E14.1TG3B1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IGK@yK2)J23.1Jkbv expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  human	IGK (50802)

• Mutation details: A 800-kb yeast artificial chromosome (YAC) was constructed from human germline sequence of the immunoglobulin kappa light chain gene cluster. This YAC consisted of 32 V-kappa gene segments, all 5 J segments, the C-kappa constant region, the downstream kappa deleting element and a neomycin resistance cassette. Tw transgenic lines were created with one, J23.1, being propagated for further analysis. Southern blot analysis confirmed that the YAC inserted without any recombination events. Transgenic mice were found to express human kappa light chain immunoglobulin. (J:70620, J:139719)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This allele is used in several Xenomouse strains.

References

• Original: J:70620 Mendez MJ, et al., Functional transplant of megabase human immunoglobulin loci recapitulates human antibody response in mice. Nat Genet. 1997 Feb;15(2):146-56
• All: 7 reference(s)