Hbb^tm4(HBB*)Tow
Targeted Allele Detail

Summary

• Symbol: Hbb^tm4(HBB*)Tow
• Name: hemoglobin beta chain complex; targeted mutation 4, Timothy Townes
• MGI ID: MGI:3802947
• Synonyms: B93A, betaC93A
• Gene: Hbb Location: unknown Genetic Position: Chr7, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137709
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Hbb^tm4(HBB*)Tow involves 2 genes/genome features (Hbb-b2, Hbb-b1) View all
• Hbb^tm4(HBB*)Tow expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBB (3043)

  Hbb-b1	(MGI:96021)
  Hbb-b2	(MGI:96022)
  Hbb-bh2	(MGI:96025)
  Hbb-bs	(MGI:5474852)
  Hbb-bt	(MGI:5474850)

  Expresses human HBB containing amino acid substitution of alanine for cysteine at position 93 of human HBB

• Mutation details: In ES cells that were homozygous for Hba^tm1(HBA)Tow and Hbb^{tm2(HBG1,HBB*)Tow} the human gamma chain and beta chain containing the sickle cell mutation were replaced with 4.6 kb fragment of human HBB containing nucleotide substitutions that lead to an amino acid substitution of alanine for cysteine at position 93 of human HBB. Flanking sequence that extends to -815 of HBB as well as a floxed hygro cassette were also inserted. Cre-mediated recombination was used to remove the hygro cassette. (J:137709)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hbb Mutation: 47 strains or lines available

References

• Original: J:137709 Isbell TS, et al., SNO-hemoglobin is not essential for red blood cell-dependent hypoxic vasodilation. Nat Med. 2008 Jul;14(7):773-7
• All: 4 reference(s)