Tnnt2^tm2Mmto
Targeted Allele Detail

Summary

• Symbol: Tnnt2^tm2Mmto
• Name: troponin T2, cardiac; targeted mutation 2, Sachio Morimoto
• MGI ID: MGI:3803301
• Synonyms: cTnT^deltaK210, Tnnt2^tm1.1Mmto
• Gene: Tnnt2 Location: Chr1:135764092-135779998 bp, + strand Genetic Position: Chr1, 59.32 cM
• Alliance: Tnnt2^tm2Mmto page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137784
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 13 was replaced with a floxed neo cassette and an exon 13 containing a deletion of three base pairs that results in the deletion of lysine at position 210 (K210). The neo cassette was removed by germ line, cre-mediated recombination. This mutation mimicks one identified in human patients with dilated cardiomyopathy. Similar levels of troponin T protein are detected in cardiac myofibrils isolated from the hearts of mice homozygous for this mutation and of wild-type mice by sodium dodecyl sulftate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis for total protein. (J:137784, J:173751)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnnt2 Mutation: 51 strains or lines available

References

• Original: J:137784 Du CK, et al., Knock-in mouse model of dilated cardiomyopathy caused by troponin mutation. Circ Res. 2007 Jul 20;101(2):185-94
• All: 14 reference(s)