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Tg(Pvalb-EGFP)B20Zjh
Transgene Detail
Summary
Symbol: Tg(Pvalb-EGFP)B20Zjh
Name: transgene insertion B20, Z Josh Huang
MGI ID: MGI:3803745
Synonyms: B20, Tg(Gad1-EGFP)B20Zjh
Transgene: Tg(Pvalb-EGFP)B20Zjh  Location: unknown  
Alliance: Tg(Pvalb-EGFP)B20Zjh page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA BAC clone containing the entire Pvalb gene and approximately 150 kb of upstream and 25 kb downstream regions was used for BAC modifications. A GFP expression cassette was inserted in the first coding exon at the translation initiation site. Five transgenic founders were identified by PCR and confirmed by southern blotting. In the cerebellum, different fractions of Purkinje, basket, and stellate neurons expressed GFP among different transgenic lines, from a few percent (the B20 line) to near 100% (the B13 line, and unpublished data), likely due to different genomic integration sites of the transgene. The onset of GFP usually started in the late second postnatal week and increased to higher levels by the fourth week. (J:136656)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:94812 Chattopadhyaya B, et al., Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. J Neurosci. 2004 Oct 27;24(43):9598-611
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory