Nicoq3^C3H/HeJ
QTL Variant Detail

Summary

• QTL variant: Nicoq3^C3H/HeJ
• Name: nicotine consumption QTL 3; C3H/HeJ
• MGI ID: MGI:3803893
• QTL: Nicoq3 Location: unknown Genetic Position: Chr7, Syntenic

Variant origin

• Strain of Specimen: C3H/HeJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased voluntary nicotine consumption compared to C57BL/6J. (J:137282)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:137282

Genetic loci associated with nicotine consumption were mapped in an F2 intercross between high and low nicotine consumption inbred mouse strains C57BL/6J and C3H/HeJ, respectively. Animals 55- to 65-days old were subjected to a 4-bottle (0, 25, 50 and 100 ug/ml nicotine solution) choice test over an 8 day period. Phenotypically extreme F2 animals (n=203) were selected for linkage analysis using 80 polymorphic markers (77 microsatellite markers and 3 SNPs) at an average resolution of 20 cM. A total of four significant QTLs were identified that, together, explain approximately 62% of the phenotypic variance.

Significant linkage to nicotine consumption mapped to 95.8 cM on mouse Chromosome 1 near D1Mit206 (LOD=15.38) and is named Nicoq1 (nicotine consumption QTL 1). C57BL/6J-derived alleles at Nicoq1 confer higher nicotine consumption. This locus explains 30% of the phenotypic variance. The distal portion of the QTL appears to have a male-specific effect; however authors indicate further study is necessary to confirm this. Previously identified alcohol QTLs named Alcw1 and Alcdp1 at 95.8 cM colocalize with Nicoq1. Human Chromosome 1 harbors potential QTL for nicotine addiction at 168 cM - 196 cM, an interval syntenic to Nicoq1.

Nicoq2 (nicotine consumption QTL 2) maps to 49.6 cM on mouse Chromosome 4 near D4Mit175 (LOD=3.93). This locus appears to be female-specific and explains 9% of the variance in nicotine consumption. Authors indicate further study is required to confirm the female-specific effect.C57BL/6J-derived alleles at Nicoq2 confer increased nicotine consumption. Previously identified alcohol withdrawal QTL Alcw2 (40 cM) overlaps with Nicoq2.

On mouse Chromosome 7, a locus for nicotine consumption named Nicoq3 (nicotine consumption QTL 3)mapped to 18 cM near D7Mit228 (LOD=5.9). C3H/HeJ-derived alleles at Nicoq3 confer increased nicotine consumption. Nicoq3 explains 13% of the phenotypic variance. Previously identified alcohol QTL Aplq at 25 cM overlaps with Nicoq3.

Nicoq4 (nicotine consumption QTL 4) mapped to 56 cM on mouse Chromosome 15 near SNP marker rs3667785 (LOD=4.47). This locus explains 10% of the phenotypic variance with C57BL/6J-derived alleles conferring increased nicotine consumption. Alprf (52.2 cM) and Vacq2 (47 cM) are previously identified alcoholQTLs mapping near Nicoq4.

Suggestive QTLs associated with nicotine consumption map to 30 cM on mouse Chromosome 14 near D14Mit39 (LOD=2.23) and 50 cM on mouse Chromosome 18 near D18Mit7 (LOD=2.62).

References

• Original: J:137282 Li XC, et al., Chromosomal loci that influence oral nicotine consumption in C57BL/6J x C3H/HeJ F2 intercross mice. Genes Brain Behav. 2007 Jul;6(5):401-10
• All: 1 reference(s)