Cul4a/Pcid2^tm2Ktc
Targeted Allele Detail

Summary

• Symbol: Cul4a/Pcid2^tm2Ktc
• Name: cullin 4A; targeted mutation 2, Kristin T Chun
• MGI ID: MGI:3805522
• Synonyms: Cul4A-flox
• Gene: Cul4a Location: Chr8:13155623-13197940 bp, + strand Genetic Position: Chr8, 5.73 cM
• Alliance: Cul4a/Pcid2^tm2Ktc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138468
• Parent Cell Line: CCE916 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of the first exon and a floxed neo cassette was inserted downstream of exon 1. Germ line, cre-mediated recombination was used to remove the neo cassette leaving exon 1 floxed. In a subsequent publication (J:150427), the authors demonstrate that that the upstream loxP site was inserted in a separate gene, Pcid2, that lies in a head-to-head configuration with Cul4a. This loxP site, inserted in a NotI restriction site, lies downstream of the putative translational start site of Pcid2. Cre-mediated excision can thus potentially disrupt both genes. (J:78032, J:138468, J:150427)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cul4a Mutation: 30 strains or lines available

References

• Original: J:138468 Waning DL, et al., Cul4A is required for hematopoietic cell viability and its deficiency leads to apoptosis. Blood. 2008 Jul 15;112(2):320-9
• All: 3 reference(s)