Fzr1^{Gt(RRJ067)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Fzr1^{Gt(RRJ067)Byg}
• Name: fizzy and cell division cycle 20 related 1; gene trap RRJ067, BayGenomics
• MGI ID: MGI:3805590
• Synonyms: Cdh1^gt
• Gene: Fzr1 Location: Chr10:81202713-81214204 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Fzr1^{Gt(RRJ067)Byg} page

Mutation origin

• Mutant Cell Line: RRJ067
• Germline Transmission: Earliest citation of germline transmission: J:138545
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The pGT0Lxf gene trap vector, containing a splice acceptor signal followed by betageo (lacZ-neo^r fusion) cDNA, has integrated into intron 5 of the gene. Immunoblot analysis of homozygous mutant E9.5 whole-embryo lysates detects no FZR1 (CDH1) protein; tissues of heterozygous mutant mice express ~50% of wild-type FZR1 levels. X-gal staining of fixed whole heterozygous E9.5 embryos reveals expression of beta-galactosidase from the gene trap insertion widely throughout the embryo and the placenta. (J:138545)
• Inheritance: Recessive
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ067	CC326219 ; (MGI Seq Detail)	5' RACE	Chr10:81206531-81207199 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  38 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fzr1 Mutation: 43 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:138545 Li M, et al., The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and memory. Nat Cell Biol. 2008 Sep;10(9):1083-9
• All: 3 reference(s)