Ins2^tm1Nagy
Targeted Allele Detail

Summary

• Symbol: Ins2^tm1Nagy
• Name: insulin II; targeted mutation 1, Andras Nagy
• MGI ID: MGI:3805819
• Synonyms: I2, Ins2^I2
• Gene: Ins2 Location: Chr7:142232393-142233463 bp, - strand Genetic Position: Chr7, 88.0 cM
• Alliance: Ins2^tm1Nagy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130399
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette in which the phosphoglycerate kinase promoter alone is flanked by loxP sites (loxP-PGK-loxP-neo-pA) was inserted 2.6 kb upstream of (distal to) and in the same orientation as the insulin 2 gene on Chromosome 7, between the domain of influence of Chr 7 imprinting center (IC) 1 (H19 DMR) and that of IC2 (KvDMR1). (J:130399)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ins2^tm1Nagy/Ins2^tm1Nagy	involves: 129S1/Sv * 129X1/SvJ * Hsd:ICR

Phenotypes:

Affected Systems	hm1
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normal phenotype	√
no abnormal phenotype detected	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ins2 Mutation: 93 strains or lines available

References

• Original: J:130399 Oh R, et al., Epigenetic and phenotypic consequences of a truncation disrupting the imprinted domain on distal mouse chromosome 7. Mol Cell Biol. 2008 Feb;28(3):1092-103
• All: 2 reference(s)