Lat^{tm6.1(HBEGF/EGFP)Mal}
Targeted Allele Detail

Summary

• Symbol: Lat^{tm6.1(HBEGF/EGFP)Mal}
• Name: linker for activation of T cells; targeted mutation 6.1, Bernard Malissen
• MGI ID: MGI:3805830
• Synonyms: Lat^DTR, Lattm6Mal
• Gene: Lat Location: Chr7:125962996-125968742 bp, - strand Genetic Position: Chr7, 69.01 cM
• Alliance: Lat^{tm6.1(HBEGF/EGFP)Mal} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138401
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, No functional change, Reporter)
• Mutation: Insertion
• Lat^{tm6.1(HBEGF/EGFP)Mal} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

  human cDNA-EGFP fusion gene inserted in 3' UTR downstream of IRES

• Mutation details: A loxP site was inserted into intron 1 and an IRES2-DTR-EGFP-loxP-Neo-loxP cassette was inserted 114 bp downstream of the stop codon in the 3' UTR in exon 12. Correctly targeted ES cells were transiently transfected with cre recombinase and clones that had just the neomycin cassette removed were selected for mouse production. This left the human diphtheria toxin receptor gene (HBEGF)-EGFP fuson gene cassette in the 3' UTR. Very low levels of EGFP were detected in thymocytes. Thymocytes and T cells from homozygous mice expressed approximately 1.2-fold less LAT molecules than those of wild-type mice. T cell function was not impaired in these mice. Cre-recombinase expression will remove most of the coding sequence and the DTR/EGFP-expression cassette from the locus. (J:138401, J:151840)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lat Mutation: 10 strains or lines available

References

• Original: J:138401 Helft J, et al., Antigen-specific T-T interactions regulate CD4 T-cell expansion. Blood. 2008 Aug 15;112(4):1249-58
• All: 3 reference(s)