Tg(tetO-SOD1*G93A,-luc)1Roos
Transgene Detail

Summary

• Symbol: Tg(tetO-SOD1*G93A,-luc)1Roos
• Name: transgene insertion 1, Raymond Roos
• MGI ID: MGI:3806292
• Synonyms: Luc:BiTet:G93ASOD1
• Transgene: Tg(tetO-SOD1*G93A,-luc)1Roos Location: unknown
• Alliance: Tg(tetO-SOD1*G93A,-luc)1Roos page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A luciferase coding sequence and cDNA encoding human SOD1 with a G93A amino acid substitution were cloned into a plasmid containing a bi-cistronic tetracycline operator promoter (BiTetO). The construct was injected into micronuclei. Five lines were generated. Transgene expression was determined by luciferase activity in isolated fibroblasts transfected with a CMV-tTA plasmid. Analysis using anti-SOD1 and -luciferase antibodies was also performed on fibroblasts. (J:138761)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:138761 Wang L, et al., Restricted expression of mutant SOD1 in spinal motor neurons and interneurons induces motor neuron pathology. Neurobiol Dis. 2008 Mar;29(3):400-8
• All: 1 reference(s)