Tg(H2-K-HLA-G,B2M)1Alm
Transgene Detail
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Symbol: |
Tg(H2-K-HLA-G,B2M)1Alm |
Name: |
transgene insertion 1, Andrew L Mellor |
MGI ID: |
MGI:3806366 |
Synonyms: |
HLA-Gtg |
Transgene: |
Tg(H2-K-HLA-G,B2M)1Alm Location: unknown
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Alliance: |
Tg(H2-K-HLA-G,B2M)1Alm page
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Transgene Type: |
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Transgenic (Humanized sequence, Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(H2-K-HLA-G,B2M)1Alm expresses
2 genes
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
B2M (567) |
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human |
HLA-G (3135) |
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Mutation details: Two transgenic constructs were co-injected into male pronuclei of CBA/Ca zygotes. The constructs encoded (i) a H-2Kb/HLA-G transgene with the human HLA-G heavy chain (HLA-G; major histocompatibility complex, class I, G) gene inserted between the mouse H-2Kb MHC class I promoter and coding sequence, and (ii) a human B2M transgene (designed with the human beta2-microglobulin gene). (A third transgene was also co-injected as well, but was found not to have integrated into the genome.) The mean copy number of HLA-G genomic DNA per haploid mouse genome was 3 copies. HLA-G expression is observed in pre-implantation embryos and a variety of tissues; expression in the reproductive organs (uterus, ovary, and testes), lung, and liver is much more similar to mouse expression of Qa-2 than to Kb (despite the presence of the H-2Kb promoter and downstream coding sequence), while HLA-G expression in spleen, thymus and kidney correlates better with Kb expression patterns.
(J:140011, J:140016)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:140011 Warner CM, et al., Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success. Reprod Biomed Online. 2008 Oct;17(4):470-85 |
All: |
2 reference(s) |
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