E2f3^tm4Gle
Targeted Allele Detail

Summary

• Symbol: E2f3^tm4Gle
• Name: E2F transcription factor 3; targeted mutation 4, Gustavo Leone
• MGI ID: MGI:3806819
• Synonyms: E2f3a^3bki, E2f3a^3bKI
• Gene: E2f3 Location: Chr13:30090558-30170046 bp, - strand Genetic Position: Chr13, 13.09 cM, cytoband A3.3
• Alliance: E2f3^tm4Gle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138289
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted 108 bp upstream of the E2f3 isoform "a" start codon, the exon 1b open reading frame replaced the exon 1a open reading frame, and a third loxP site was inserted approximately 300 bp downstream of exon 1a. Western blot analysis of MEFs confirmed the absence of expression of the "a" isoform with retention of expression of the "b" isoform. (J:138289)

Tumor Data

List all tumor models in MMHCdb carrying E2f3^tm4Gle

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any E2f3 Mutation: 33 strains or lines available

References

• Original: J:138289 Tsai SY, et al., Mouse development with a single E2F activator. Nature. 2008 Aug 28;454(7208):1137-41
• All: 2 reference(s)