Bpnt2^{Gt(KST245)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Bpnt2^{Gt(KST245)Byg}
• Name: 3'(2'), 5'-bisphosphate nucleotidase 2; gene trap KST245, BayGenomics
• MGI ID: MGI:3806990
• Synonyms: Jaws^-
• Gene: Bpnt2 Location: Chr4:4762484-4793306 bp, - strand Genetic Position: Chr4, 2.56 cM
• Alliance: Bpnt2^{Gt(KST245)Byg} page

Mutation origin

• Mutant Cell Line: KST245
• Germline Transmission: Earliest citation of germline transmission: J:137353
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: Insertion of the gene-trap vector into the third of four introns is predicted to result in the first 213 amino acids of the JAWS protein fused in-frame with a beta-geo reporter. Less than 0.2% expression of wild-type (WT) transcripts and negligible expression of the endogenous protein is seen. (J:137353)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST245	CC144727 ; (MGI Seq Detail)	5' RACE	Chr4:4776313-4778268 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bpnt2 Mutation: 18 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:137353 Sohaskey ML, et al., JAWS coordinates chondrogenesis and synovial joint positioning. Development. 2008 Jul;135(13):2215-20
• All: 2 reference(s)