Rsph1^tm1Htan
Targeted Allele Detail

Summary

• Symbol: Rsph1^tm1Htan
• Name: radial spoke head 1 homolog (Chlamydomonas); targeted mutation 1, Hiromitsu Tanaka
• MGI ID: MGI:3807704
• Gene: Rsph1 Location: Chr17:31473993-31496270 bp, - strand Genetic Position: Chr17, 15.94 cM
• Alliance: Rsph1^tm1Htan page

Sperm are sparse or absent in Rsph1^tm1Htan/Rsph1^tm1Htan epididymides and sometimes have abnormal heads

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138122
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1-3 were replaced by a neomycin cassette. mRNA was not detectable in homozygous mice by Northern blo analysis and protein was not detectable by Western blo in extracts of testes. (J:138122)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rsph1 Mutation: 23 strains or lines available

References

• Original: J:138122 Tokuhiro K, et al., Meichroacidin containing the membrane occupation and recognition nexus motif is essential for spermatozoa morphogenesis. J Biol Chem. 2008 Jul 4;283(27):19039-48
• All: 2 reference(s)