Tal1^tm1Por
Targeted Allele Detail

Summary

• Symbol: Tal1^tm1Por
• Name: T cell acute lymphocytic leukemia 1; targeted mutation 1, Catherine Porcher
• MGI ID: MGI:3808120
• Synonyms: scl^RER
• Gene: Tal1 Location: Chr4:114913623-114928952 bp, + strand Genetic Position: Chr4, 52.73 cM
• Alliance: Tal1^tm1Por page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139179
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Three highly conserved DNA-binding residues encoded in exon 6 (Arg-Glu-Arg at positions 195-197) were mutated to alanine residues. A floxed neo cassette was also inserted upstream of exon 6. Transient expression of Cre recombinase excised the neo cassette leaving behind a loxP site. The mutant protein was expressed at endogenous levels in the fetal liver, was localized to cellular nuclei, but lacked the ability to bind DNA. (J:139179)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tal1 Mutation: 33 strains or lines available

References

• Original: J:139179 Kassouf MT, et al., Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis. Blood. 2008 Aug 15;112(4):1056-67
• All: 1 reference(s)