Cyp4v3^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Cyp4v3^{tm1(KOMP)Vlcg}
• Name: cytochrome P450, family 4, subfamily v, polypeptide 3; targeted mutation 1, Velocigene
• MGI ID: MGI:3808361
• Synonyms: Cyp4v3^tm1Vlcg
• Gene: Cyp4v3 Location: Chr8:45758838-45786200 bp, - strand Genetic Position: Chr8, 25.17 cM
• Alliance: Cyp4v3^{tm1(KOMP)Vlcg} page
• IMPC: Cyp4v3 gene page

Mutation origin

• Mutant Cell Lines: 10578B-B5, 10578B-C11, 10578B-D8, 10578B-F9, 10578B-G3
• Germline Transmission: Earliest citation of germline transmission: J:230232
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 26040bp between positions 45759973-45786012 of Chromosome 8 (Build GRCm39). The cassette replace from part of exon 1 to part of exon 11. Western blot analysis confirmed absence of protein. (J:136110, J:230232)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Cyp4v3 Mutation: 36 strains or lines available

Notes

Cell line 10578B-B5 was successfully used to make chimeric mice. Germline transmission was accomplished. J:230232.

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 2 reference(s)