G2e3^{Gt(RRM192)Byg}
Gene trapped Allele Detail

Summary

• Symbol: G2e3^{Gt(RRM192)Byg}
• Name: G2/M-phase specific E3 ubiquitin ligase; gene trap RRM192, BayGenomics
• MGI ID: MGI:3808723
• Synonyms: G2E3^-
• Gene: G2e3 Location: Chr12:51395013-51423769 bp, + strand Genetic Position: Chr12, 22.11 cM
• Alliance: G2e3^{Gt(RRM192)Byg} page

Mutation origin

• Mutant Cell Line: RRM192
• Germline Transmission: Earliest citation of germline transmission: J:139062
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutations: Insertion of gene trap vector, Intragenic deletion Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector is inserted in intron 13, about 570 base pairs downstream from the end of exon 13. Insertion also resulted in the deletion of 64 nucleotides from intron 13 just upstream from the insertion site. (J:139062)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRM192	CG672132 ; (MGI Seq Detail)	5' RACE	Chr12:51412152-51414740 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  29 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

42 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any G2e3 Mutation: 35 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:139062 Brooks WS, et al., G2E3 is a dual function ubiquitin ligase required for early embryonic development. J Biol Chem. 2008 Aug 8;283(32):22304-15
• All: 2 reference(s)