Fen1^tm2Klng
Targeted Allele Detail

Summary

• Symbol: Fen1^tm2Klng
• Name: flap structure specific endonuclease 1; targeted mutation 2, Arne Klungland
• MGI ID: MGI:3808779
• Synonyms: Fen1^E160D
• Gene: Fen1 Location: Chr19:10176496-10181533 bp, - strand Genetic Position: Chr19, 6.54 cM
• Alliance: Fen1^tm2Klng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138901
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Site directed mutagenesis followed by homologous recombination was used to modify the coding sequence of exon 2 creating an amino acid substitution of aspartic acid for glutamic acid at position 160 (E160D). This mutation alters the single active residue site in the nuclease core domain. A neo cassette was also inserted downstream of exon 2 for selection purposes. (J:138901)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fen1 Mutation: 32 strains or lines available

References

• Original: J:138901 Larsen E, et al., Early-onset lymphoma and extensive embryonic apoptosis in two domain-specific Fen1 mice mutants. Cancer Res. 2008 Jun 15;68(12):4571-9
• All: 2 reference(s)