Vwa1^tm1Jfba
Targeted Allele Detail

Summary

• Symbol: Vwa1^tm1Jfba
• Name: von Willebrand factor A domain containing 1; targeted mutation 1, John F Bateman
• MGI ID: MGI:3809230
• Synonyms: Vwa1^betagal
• Gene: Vwa1 Location: Chr4:155852952-155859042 bp, - strand Genetic Position: Chr4, 87.31 cM, cytoband E2
• Alliance: Vwa1^tm1Jfba page

Collagen VI matrix is disorganized and reduced in the Vwa1^tm1Jfba/Vwa1^tm1Jfba sciatic nerve endoneurial matrix

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139369
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: The open reading frame was replaced LacZ gene with a nuclear localization signal and a floxed neo cassette. The expression of the reporter gene from the Vwa1 locus in heterozygous mice recapitulated gene expression in wild type mice. Northern blot analysis of total RNA extracted from spinal cord and brain demonstrated that the gene transcript is undetectable in homozygous mutant mice. The encoded protein was undetectable in nerve lysates by immunoblotting and in sections of sciatic nerve by immunofluorescence staining. (J:139369, J:149746)

Generation of the Vwa1^tm1Jfba allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vwa1 Mutation: 38 strains or lines available

References

• Original: J:139369 Allen JM, et al., The extracellular matrix protein WARP is a novel component of a distinct subset of basement membranes. Matrix Biol. 2008 May;27(4):295-305
• All: 2 reference(s)