Csrp2^tm1Rawe
Targeted Allele Detail

Summary

• Symbol: Csrp2^tm1Rawe
• Name: cysteine and glycine-rich protein 2; targeted mutation 1, Ralf Weiskirchen
• MGI ID: MGI:3809532
• Gene: Csrp2 Location: Chr10:110756037-110775375 bp, + strand Genetic Position: Chr10, 57.84 cM
• Alliance: Csrp2^tm1Rawe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138969
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neo cassette was inserted into exon 4 at nucleotide 7292 via homologous recombination. Northern blot and quantitative RT-PCR confirmed a 50% reduction in wild-type transcript and detected an aberrant message consisting of a splicing between exon 3 and the downstream neo cassette/exon 4 boundary in heterozygotes. Western blot analysis of kidney homogenates from homozygotes confirmed the absence of protein. (J:138969)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Csrp2 Mutation: 95 strains or lines available

References

• Original: J:138969 Sagave JF, et al., Targeted disruption of the mouse Csrp2 gene encoding the cysteine- and glycine-rich LIM domain protein CRP2 result in subtle alteration of cardiac ultrastructure. BMC Dev Biol. 2008;8:80
• All: 1 reference(s)