Cd83^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Cd83^tm1Mak
• Name: CD83 antigen; targeted mutation 1, Tak W Mak
• MGI ID: MGI:3809654
• Synonyms: CD83EGFP
• Gene: Cd83 Location: Chr13:43938661-43956608 bp, + strand Genetic Position: Chr13, 21.6 cM, cytoband A4-5
• Alliance: Cd83^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138988
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A reporter cassette consisting of eGFP gene linked to a viral IRES2 sequence was positioned right after the stop codon located in exon 5 of the gene. A neomycin resistance gene was included after the EGFP stop codon. The entire coding region of the gene remained intact in the targeting construct. Expression of the targeted gene generated a bicistronic transcript encoding both CD83 and eGFP. (J:138988)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd83 Mutation: 17 strains or lines available

References

• Original: J:138988 Lechmann M, et al., The CD83 reporter mouse elucidates the activity of the CD83 promoter in B, T, and dendritic cell populations in vivo. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11887-92
• All: 2 reference(s)