Tg(Foxp3-EGFP/cre)1cJbs
Transgene Detail
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Symbol: |
Tg(Foxp3-EGFP/cre)1cJbs |
Name: |
transgene insertion 1c, Jeffrey Bluestone |
MGI ID: |
MGI:3809724 |
Synonyms: |
FoxP3-GFP-hCre, FoxP3-GFP-hCre BAC Tg |
Transgene: |
Tg(Foxp3-EGFP/cre)1cJbs Location: unknown
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Alliance: |
Tg(Foxp3-EGFP/cre)1cJbs page
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Transgene Type: |
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Transgenic (Recombinase) |
Mutation: |
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Insertion
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Tg(Foxp3-EGFP/cre)1cJbs expression driven by
1 gene
Transgene expression driven by:
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Mutation details: An EGFP-h-Cre cDNA (GFP fused to a codon-optimized, "humanized" cre recombinase sequence) was inserted directly downstream of the Foxp3 ATG start codon by homologous recombination in a 188-kb C57BL/6 BAC clone (RP23-143D8) containing the entire Foxp3 gene. The purified BAC was injected into pronuclei of nonobese, diabetic mouse embryos. Several founders were generated. GFP expression and lack of functional Foxp3 protein, as indicated by inability of the transgene to rescue Foxp3 null mice were analyzed and line 1 was established. Functional cre activity was assayed by breeding mice to Gt(ROSA)26Sortm1(EYFP)Cos reporter mice, and high specificity of recombination was detected in the lymph nodes and spleen. This is the second of two lines derived from founder 1. Cre mediated recombination and GFP expression is less than in line 1a.
(J:138808)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Foxp3
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:157603 Bluestone J, A low expressing line derived from the same founder as the previously reported Tg(Foxp3-EGFP/cre)1aJbs high expressing transgene line. MGI Direct Data Submission. 2010; |
All: |
55 reference(s) |
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