Ube2k^Gt(XK109)Byg
Gene trapped Allele Detail

Summary

• Symbol: Ube2k^Gt(XK109)Byg
• Name: ubiquitin-conjugating enzyme E2K; gene trap XK109, BayGenomics
• MGI ID: MGI:3809822
• Synonyms: E2-25K/Hip-2^-
• Gene: Ube2k Location: Chr5:65694588-65756332 bp, + strand Genetic Position: Chr5, 33.72 cM
• Alliance: Ube2k^Gt(XK109)Byg page

Mutation origin

• Mutant Cell Line: XK109
• Germline Transmission: Earliest citation of germline transmission: J:138978
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector pGT0Lxf was inserted into intron 1. Reduced protein expression was confirmed by western blot analysis on brain extracts. (J:138978)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XK109	CC248840 ; (MGI Seq Detail)	5' RACE	Chr5:65694745-65694886 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  167 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube2k Mutation: 69 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:138978 Song S, et al., E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity. J Cell Biol. 2008 Aug 25;182(4):675-84
• All: 3 reference(s)