Lbr^Gt(XE569)Byg
Gene trapped Allele Detail

Summary

• Symbol: Lbr^Gt(XE569)Byg
• Name: lamin B receptor; gene trap XE569, BayGenomics
• MGI ID: MGI:3809824
• Synonyms: Lbr^GT
• Gene: Lbr Location: Chr1:181642880-181669933 bp, - strand Genetic Position: Chr1, 84.89 cM
• Alliance: Lbr^Gt(XE569)Byg page

Mutation origin

• Mutant Cell Line: XE569
• Germline Transmission: Earliest citation of germline transmission: J:138854
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The pGT1Lxf gene trap vector inserted into exon 9. Northern blot analysis detects a larger fusion transcript in addition to the wild-type transcript in heterozygous fibroblasts. A fusion polypeptide consisting of the amino terminal nucleoplasmic domain and first four transmembrane domains fused to beta-geo is detected by immunofluorescence in PMEFs. (J:138854)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE569	CC183148 ; (MGI Seq Detail)	5' RACE	Chr1:181649001-181656390 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  18 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lbr Mutation: 57 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:138854 Cohen TV, et al., The lamin B receptor under transcriptional control of C/EBPepsilon is required for morphological but not functional maturation of neutrophils. Hum Mol Genet. 2008 Oct 1;17(19):2921-33
• All: 3 reference(s)