Tg(IGH@yHG1/2)1Amgn
Transgene Detail

Summary

• Symbol: Tg(IGH@yHG1/2)1Amgn
• Name: transgene insertion 1, Amgen
• MGI ID: MGI:3810128
• Synonyms: yHG1/2
• Transgene: Tg(IGH@yHG1/2)1Amgn Location: unknown
• Alliance: Tg(IGH@yHG1/2)1Amgn page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:139792
• Parent Cell Line: E14.1TG3B1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IGH@yHG1/2)1Amgn expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  human	IGH (3492)

• Mutation details: A yeast artificial chromosome (YAC) was constructed from human germline sequence of the immunoglobulin heavy chain gene cluster. This YAC consisted of 66 V-heavy gene segments, all 30 D gene segments, all 6 J segments, the C-mu, and the C-delta gene segments, the human gamma2 class switch recombination recognition site, followed by the gamma 1 constant region. Downstream of this, the human exons encoding the membrane sequence of gamma2 constant regions were introduced followed by a downstream murine enhancer element. Southern blot analysis confirmed that the YAC inserted into ES cells without any recombination events. Mice carrying this transgene in combination with other alleles are capable of producing high yields of human IgG1 to an immunized antigen. (J:139792)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The Xenomouse HG1/2 transgenic strain contains this allele along with the Igh-J^tm1Jkbv, Igk-C^tm1Jkbv, Tg(IGK@yK2)J23.1Jkbv alleles.

References

• Original: J:139792 Green LL, et al., Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions. MGI Direct Data Submission. 2008
• All: 1 reference(s)