Del(7Or2h1-Rr320)1Mkg
Targeted Allele Detail

Summary

• Symbol: Del(7Or2h1-Rr320)1Mkg
• Name: deletion, Chr 7, Mark Groudine 1
• MGI ID: MGI:3810287
• Synonyms: Del(7Olfr64-Hbb-ar)1Mkg, Del(7Or2h1-Hbb-ar)1Mkg, deltaHS-62.5-deltaLCR
• Gene: Del(7Or2h1-Rr320)1Mkg Location: unknown Genetic Position: Chr7, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139198
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Intergenic deletion, Intragenic deletion
• Del(7Or2h1-Rr320)1Mkg involves 4 genes/genome features (Rr320, Or51b4, Or51b17 ...) View all
• Mutation details: ES cells carrying the Rr320^tm1.1Mkg allele, which has most of the Rr320 (Hbb-ar) region replaced with a loxP site, were targeted in cis with a construct that replaced the upstream HS-62.5 element with a loxP site flanked neomycin selection cassette. This substitution removed nucleotides -63238 through -59669, relative to the start site of the Hbb-y gene. Transient expression of Cre recombinase eliminated the entire genomic sequence between the newly inserted floxed neo cassette and the loxP site present from the Rr320r^tm1.1Mkg allele. This deletion removes genes Olfr64, Olf65, Olf66, and almost all of the Hbb-ar region. (J:139198)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(7Or2h1-Rr320)1Mkg Mutation: 0 strains or lines available

Notes

Male ES cells, deltaLCR-D/S-1, were generated from male mice on a 129 background that were heterozygous for the Hbb-ar^tm1.1Mkg allele, carried two different variants of the Hbb genes (Hbb^D/Hbb^S) and were hemizygous for the null Hprt^b-m3 allele.

References

• Original: J:139198 Bender MA, et al., Flanking HS-62.5 and 3' HS1, and regions upstream of the LCR, are not required for beta-globin transcription. Blood. 2006 Aug 15;108(4):1395-401
• All: 1 reference(s)