Orai1^tm1Rao
Targeted Allele Detail

Summary

• Symbol: Orai1^tm1Rao
• Name: ORAI calcium release-activated calcium modulator 1; targeted mutation 1, Anjana Rao
• MGI ID: MGI:3810487
• Gene: Orai1 Location: Chr5:123153137-123168519 bp, + strand Genetic Position: Chr5, 62.72 cM
• Alliance: Orai1^tm1Rao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139730
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 1 was replaced with a self-deleting neomycin cassette that was excised in the germ line of male mice carrying the targeted allele. Gene inactivation was confirmed by a lack mRNA transcript in CD4⁺ T cells and B cells. No protein expression was detected in the skin by immunohistochemical analysis. (J:139730)

Expression

In Mice Carrying this Mutation:	40 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Orai1 Mutation: 22 strains or lines available

Notes

ES cell line = B6/3.

References

• Original: J:139730 Gwack Y, et al., Hair loss and defective T- and B-cell function in mice lacking ORAI1. Mol Cell Biol. 2008 Sep;28(17):5209-22
• All: 10 reference(s)