Gpx4^tm2Marc
Targeted Allele Detail

Summary

• Symbol: Gpx4^tm2Marc
• Name: glutathione peroxidase 4; targeted mutation 2, Marcus Conrad
• MGI ID: MGI:3810783
• Synonyms: Gpx4^flox
• Gene: Gpx4 Location: Chr10:79883000-79892273 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Gpx4^tm2Marc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139668
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exons 5-7 encoding the Sec-insertion sequence element and the polyadenylation signal were flanked by loxP sites. A frt-flanked neomycin resistance cassette was placed immediately downstream of the 3' loxP site. Founder mice were crossed with FLPe deleter mice to remove the neomycin cassette leaving behind a single frt site. Correct targeting was confirmed by Southern blotting. (J:139668)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpx4 Mutation: 82 strains or lines available

References

• Original: J:139668 Seiler A, et al., Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death. Cell Metab. 2008 Sep;8(3):237-48
• All: 17 reference(s)