Tg(CAG-lacZ,-Akt1*,-EGFP)56Ebm
Transgene Detail

Summary

• Symbol: Tg(CAG-lacZ,-Akt1*,-EGFP)56Ebm
• Name: transgene insertion 56, Ernesto Bernal-Mizrachi
• MGI ID: MGI:3811006
• Synonyms: LSL-myr-Akt, pCALL2-caAkt
• Transgene: Tg(CAG-lacZ,-Akt1*,-EGFP)56Ebm Location: unknown
• Alliance: Tg(CAG-lacZ,-Akt1*,-EGFP)56Ebm page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:136227
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: This transgene consists of a chicken beta-actin promoter with the upstream cytomegalovirus enhancer (pCAGGS) followed by a floxed lacZ neo fusion cassette with three SV40 poladenylation sites, a constitutively active form of Akt1 with an HA epitope, an IRES site, and enhanced GFP. Cre-mediated recombination allows for expression of the constitutively active Akt1 and EGFP. Two lines were generated (42 and 56) that both had strong homogeneous lacZ expression. This is a representative record for these two lines. (J:136227)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:136227 Elghazi L, et al., Generation of a reporter mouse line expressing Akt and EGFP upon Cre-mediated recombination. Genesis. 2008 May;46(5):256-64
• All: 4 reference(s)