Gjb4^tm1Kwi
Targeted Allele Detail

Summary

• Symbol: Gjb4^tm1Kwi
• Name: gap junction protein, beta 4; targeted mutation 1, Klaus Willecke
• MGI ID: MGI:3811558
• Synonyms: Cx30.3^-, Cx30.3^LacZ
• Gene: Gjb4 Location: Chr4:127244879-127247874 bp, - strand Genetic Position: Chr4, 61.51 cM, cytoband D1-D3
• Alliance: Gjb4^tm1Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140005
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ gene with a nuclear localization signal followed by a frt-flanked neomycin selection cassette in the reverse transcriptional orientation was inserted into the starting codon of the gene locus. Founder mice were crossed with a FLP-deleter strain to remove the neomycin selection cassette. Gene inactivation was confirmed by immunoblot analysis of skin lysates. LacZ expression was detected in the skin, kidney, and a subpopulation of cells in the apical layer of the vomeronasal organ. (J:140005)

Expression

In Mice Carrying this Mutation:

44 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gjb4 Mutation: 17 strains or lines available

References

• Original: J:140005 Zheng-Fischhofer Q, et al., Characterization of connexin30.3-deficient mice suggests a possible role of connexin30.3 in olfaction. Eur J Cell Biol. 2007 Dec;86(11-12):683-700
• All: 1 reference(s)