Nct1/Nct2^tm1Nbh
Targeted Allele Detail

Summary

• Symbol: Nct1/Nct2^tm1Nbh
• Name: non-coding in testis 2; targeted mutation 1, Norman B Hecht
• MGI ID: MGI:3811745
• Synonyms: Nct1/2, Nct2^tm1Nbh
• Gene: Nct2 Location: Chr2:92379645-92380484 bp, + strand
• Alliance: Nct1/Nct2^tm1Nbh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139921
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP-flanked neomycin selection cassette replaced the entire coding sequence of the two gene loci. PicoRNAs (piRNA) from the gene loci were not detectable by real-time PCR on RNA from the testes of homozygous mice. Also, piRNAs up to 7 kb upstream (piR-135965) and 8 kb downstream of the deleted region (piR-111581) were markedly decreased in the testes of male homozygotes. Expression of the L1 retrotransposon, a target of some of these piRNAs, was increased in the testes of these mice. (J:139921)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nct2 Mutation: 0 strains or lines available

References

• Original: J:139921 Xu M, et al., Mice deficient for a small cluster of Piwi-interacting RNAs implicate Piwi-interacting RNAs in transposon control. Biol Reprod. 2008 Jul;79(1):51-7
• All: 1 reference(s)