Itsn1^tm1.1Mapr
Targeted Allele Detail

Summary

• Symbol: Itsn1^tm1.1Mapr
• Name: intersectin 1 (SH3 domain protein 1A); targeted mutation 1.1, Melanie A Pritchard
• MGI ID: MGI:3812034
• Synonyms: Itsn1-TK^-
• Gene: Itsn1 Location: Chr16:91526198-91717479 bp, + strand Genetic Position: Chr16, 53.28 cM, cytoband C4
• Alliance: Itsn1^tm1.1Mapr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140030
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette with a 5' loxP site was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. Transient cre expression in ES cells was used to removed the neo cassette and exon 3 causing a frame shift and premature termination of translation. The absence of the short and long forms of the protein was confirmed by western blot analysis on brain and heart extracts. (J:140030)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itsn1 Mutation: 101 strains or lines available

References

• Original: J:140030 Yu Y, et al., Mice deficient for the chromosome 21 ortholog Itsn1 exhibit vesicle-trafficking abnormalities. Hum Mol Genet. 2008 Nov 1;17(21):3281-90
• All: 10 reference(s)