Itsn1^tm2.1Mapr
Targeted Allele Detail

Summary

• Symbol: Itsn1^tm2.1Mapr
• Name: intersectin 1 (SH3 domain protein 1A); targeted mutation 2.1, Melanie A Pritchard
• MGI ID: MGI:3812035
• Synonyms: Itsn1-LK^-
• Gene: Itsn1 Location: Chr16:91526198-91717479 bp, + strand Genetic Position: Chr16, 53.28 cM, cytoband C4
• Alliance: Itsn1^tm2.1Mapr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140030
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette with a 5' loxP site was inserted upstream of exon 32 and an additional loxP site was inserted downstream of exon 32. Transient cre expression in ES cells was used to removed the neo cassette and exon 32 causing a frame shift and premature termination of translation. The absence of the long form of the protein was confirmed by western blot analysis on brain and heart extracts. (J:140030)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itsn1 Mutation: 101 strains or lines available

References

• Original: J:140030 Yu Y, et al., Mice deficient for the chromosome 21 ortholog Itsn1 exhibit vesicle-trafficking abnormalities. Hum Mol Genet. 2008 Nov 1;17(21):3281-90
• All: 3 reference(s)