Foxp3^tm1(cre)Saka
Targeted Allele Detail

Summary

• Symbol: Foxp3^tm1(cre)Saka
• Name: forkhead box P3; targeted mutation 1, Shimon Sakaguchi
• MGI ID: MGI:3812203
• Synonyms: FIC, Fox-IRES-Cre, Foxp3^IRES-Cre
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^tm1(cre)Saka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140085
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Foxp3^tm1(cre)Saka expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxp3 (MGI:1891436)

• Mutation details: An IRES-cre cassette and a frt-flanked neomycin resistance marker were placed into the last exon of the gene immediately after the TGA stop codon. Hemizygous male mice had slightly lower amounts of the FoxP3 gene product than controls, as determined by flow cytometry on freshly isolated lymphocytes. Crossing with cre-reporter transgenic mice demonstrates that cre expression was confined to about 15% of CD4+ T cells and ~1.5% of CD8+ T cells. (J:140085)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxp3 (mouse) Summary of all recombinase alleles driven by Foxp3. Alliance mouse Foxp3 gene page

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 58 strains or lines available

References

• Original: J:140085 Wing K, et al., CTLA-4 control over Foxp3+ regulatory T cell function. Science. 2008 Oct 10;322(5899):271-5
• All: 30 reference(s)