Tg(SV40-Igh-64C8)H3Hon
Transgene Detail

Summary

• Symbol: Tg(SV40-Igh-64C8)H3Hon
• Name: transgene insertion H3, Tasuku Honjo
• MGI ID: MGI:3813628
• Synonyms: H3
• Transgene: Tg(SV40-Igh-64C8)H3Hon Location: unknown
• Alliance: Tg(SV40-Igh-64C8)H3Hon page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(SV40-Igh-64C8)H3Hon expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Igh (MGI:96442)

• Mutation details: This transgene consists of a 1.3 kb fragment containing promoter and initiation sequence from SV40, a 0.4 kb cDNA fragment encoding the immunoglobulin heavy chain from the 4C8 hybridoma, a 1.8 kb fragment containing Igh-J3 and Igh-J4 and enhancer sequences, and a 12 kb fragment containing the major intron and Igh-6 from the genomic clones Ch-M-Ig-mu-18 and lambda gtWES-IgH714, respectively. The 4C8 hybridoma contains immunoglobulin genes that encode an anti-murine erythrocyte autoantibody. Seven lines were generated, this a representative record for all lines. The H3 line carries about 10 copies of the transgene on an autosome. RNase protection analysis confirmed expression of the transgene in lymphoid tissues. (J:139864)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:139864 Okamoto M, et al., A transgenic model of autoimmune hemolytic anemia. J Exp Med. 1992 Jan 1;175(1):71-9
• All: 2 reference(s)