Kras^tm5Tyj
Targeted Allele Detail

Summary

• Symbol: Kras^tm5Tyj
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 5, Tyler Jacks
• MGI ID: MGI:3813896
• Synonyms: K-ras FSF, K-ras^FSF-G12D
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm5Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140469
• Parent Cell Line: V26.2 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: This allele has a FRT-STOP-FRT cassette in intron 1 of the oncogenic form of the gene (p.G12D mutation). Before expression of enhanced Flp (FLPe) or mouse codon-optimized FLP (FLPo) recombinase, the allele is not expressed due to the transcriptional STOP cassette. Following introduction of the recombinase, the STOP cassette is removed resulting in expression of the mutant Kras transcript and protein. (J:140469)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:140469 Jacks T, Generation of a conditional mutant Kras allele. MGI Direct Data Submission. 2008
• All: 30 reference(s)